Antibodies
Monoclonal Antibody to PI-3 Kinase, human, recombinant (mouse), Clone: AB6, Isotype: Mouse, IgG1k . Application: Western blot, Immunocytochemistry, Immunoprecipitation, Cross Reactivity with other species has not been determined, Immunogen: recombinant human p85a expressed in E. coli.
Product Number: P-1145-I
Reacts with p85a which is the regulatory subunit of PI 3-kinase. Does not react with p85b .
100 mg $310.00 Order Here
Monoclonal Antibody to phosphatidylinositol-3,4,5-trisphosphate, [Anti-PtdIns(3,4,5)P3],
Product Number: P-1173-I
Purified mouse monoclonal IgM, from in-vitro production.
Antibody Specificity: Shown to recognize PtdIns(3,4,5)P3 in murine and human cells. Crossreactivity is 1% or less with other phosphatidylinositol phosphates or lipids.
Concentration: 1.0 mg/ml in 100 mM sodium bicarbonate +0.02% sodium azide
Storage: Store at 2-8°. For extended storage add equal volume of glycerol to make a final glycerol concentration of 50% followed by storage at -20°C. Do not freeze-thaw antibody. Purification: Ultrafiltration, Blue Sepharose and Sepharose Q. Immunogen: Synthetic PtdIns(3,4,5)P3 conjugated to BSA. Suggested Usage: 1:50 for fluorescence immunohistochemistry or cytochemistry. A detailed procedure for visualization of PtdIns(3,4,5)P3 in PDGF or insulin-stimulated, permeabilized cells using biotinylated anti-mouse IgM and a fluorescent streptavidin is available. here for Product Data Sheet
Use this Product with: D-myo-phosphatidylinositol 3,4,5-trisphosphates, PtdIns(3,4,5)P3
100 ul $240.00 Order
Monoclonal Antibody to Phosphatidylinositol-4,5,-biphosphate, Purified, Anti-PtdIns(4,5)P2,
Clone: 2C11. Immunogen: Synthetic PtdIns(4,5)P2 (1) in liposomes. Specificity: Shown to recognize PtdIns(4,5)P2 in murine and human cells. Application: 1:500 for dot blot. 1:250 for TLC overlay and ELISA. 1:50 IHC, IF.
Prod.# P-1219-I
100 ul $290.00 Order
Store at 2-8 C for up to 30 days. If longer storage is necessary, aliquot and freeze at -20°C. Do not freeze-thaw antibody. Store in Tightly Sealed Vial.
Polyclonal Antibody to Inositol 1,4,5-Triphosphate Receptor Type I, (IP3 Receptor Type I)
Host: Rabbit. Immunogen: Human IP3R-I (1829-1848) peptide sequence. Specificity: Recognizes ~240 kDa IP3 receptor type I in human, dog, and rat. Application: WB 1/1000, IH 1/100.
Product Number: I-1064-I
100 ul $280.00 Order
Polyclonal Antibody to Inositol 1,4,5-trisphosphate receptor type-II, (IP3 Receptor Type II) Rabbit Polyclonal Antibody. Immunogen: Synthetic peptide: E(317) L N P D Y R D A Q N E G K T V R D(334). Specificity: Detects inositol 1,4,5-trisphosphate receptor type-II (IP3R-II) from rat tissues. IP3R-II is found predominantly in the brain. Application: IHC, no WB. IHC of hippocampus, corpus collosum, and cerebellum tissue has been tested.
Product Number: I-1092-I
100 ul $225.00 Order
Polyclonal Antibody to Inositol 1,4,5-trisphosphate receptor type-III (IP3R-III),
Host: Rabbit Immunogen: Synthetic peptide: R(2656) Q R L G F V D V Q N C M S R(2670). Specificity: Detects inositol 1,4,5-trisphosphate receptor type-III (IP3R-III) from rat tissues. IP3R-III is known to be expressed in pancreatic islets, kidney, and the gastrointestinal tract. Application: IHC, no WB.
Product Number: I-1094-I
100 ul $225.00 Order
Antiserum to PTEN,
Prod.# P-1191-I
From Rabbit. Immunogen: Raised against a fusion protein containing the C-terminal human PTEN sequence. Specificity: Detects a ~60 kDa band of human, mouse and rat PTEN by Western Blot
Research Applications: Immunoblotting:1:1000 Dilution, Immunoprecipitation: 1-2 ul/sample, Lipid Phosphatase Assay: Details available, IP-Phosphatase Assay: Details available.
PTEN plays an important role in the modulation of the 1-phosphatidylinositol 3-kinase pathway, by catalyzing the degradation of the PtdIns(3,4,5)P3 generated by PtdIns 3-kinase. This inhibits downstream functions mediated by PtdIns 3-kinase pathway.
100 ul $320.00 Order
1. Cantley, L.C. & B.G. Neel. New Insights into Tumor Suppression: PTEN Suppresses Tumor Formation by Restraining the Phosphoinositide 3-Kinase / AKT Pathway. PNAS. 1999. 96: 4240.
2. Maehama, T., & J.E. Dixon. PTEN: A Tumour Suppressor that Functions as a Phospholipid Phosphatase. Trends Cell Biol. 1999 9: 125.
3. Di Cristofano, A., & P.P. Pandolfi. The Multiple Roles of PTEN in Tumor Suppression. Cell. 2000. 100. 387.
Monoclonal Antibody to PTEN, Purified
Clone: 6H2.1. Isotype: Mouse IgG. Immunogen: Raised against a fusion protein containing the C-terminal human PTEN sequence. Specificity: Detects a ~60 kDa band in total cell lysates.
Research Applications: Immunoblotting:1-5 ug/ml, Immunoprecipitation: 5 ug/sample, Immunohistochemistry: yes, Immunofluorescence: yes, ELISA: yes.
Prod.# P-1195-I
PTEN plays an important role in the modulation of the 1-phosphatidylinositol 3-kinase pathway. PTEN inhibits downstream functions mediated by PtdIns 3-kinase pathway such as activation of PKB, cell survival and cell proliferation.
100 ug
$450.00
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Overview
Phosphoinositides play a central role in the regulation of numerous cell signaling processes. Their invovlement in cytoskeletal remodeling, vesicle trafficking, cell survival, mitogenesis, DNA repair, transcription regulation, exo-endocytosis, ion channel modulation, phagocytosis, and apoptosis have been reported.
Phosphoinositides are a form of lipids that have two fatty acid chains that insert into the membrane, and a water-soluble inositol ring head group that sticks out of the membrane into the cell. This inositol ring can be quickly modified via phosphorylation or dephosphorylation. This phosphorylation-dephosphorylation of phosphoinositides represents a mechanism for regulation signaling complexes and for the regulation of membrane proteins. The inositol ring can be phosphorylated at numerous positions and each of the resulting molecules can be recognized by specific proteins, enabling swift and accurate communication in the cell.
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